Table 2.
Biophysical properties of wild‐type and variant KCNC1.
| Group | n | Peak current density (+40 mV) | Tail current (−40 mV) | Voltage dependence of activation (V 1/2) | Slope factor | Time constant of activation | Time constant of deactivation | Resting membrane potential (V m ) |
|---|---|---|---|---|---|---|---|---|
| pA/pF | mV | k | t (ms) | t (ms) | mV | |||
| WT | 11 | 1564 ± 211 | ‐2 ± 2 | 20.2 ± 0.6 | 0.034 ± 0.001 | 0.59 ± 0.06 | 3.9 ± 0.2 | −38.8 ± 2.4 |
| Met430Ile | 9 | 1143 ± 196 | 134 ± 28*** | 3.2 ± 1.9*** | 0.021 ± 0.001*** | 0.46 ± 0.05 | 9.1 ± 1.1*** | −85.7 ± 3.5*** |
| Val432Met | 16 | 1736 ± 257 | 270 ± 53*** | −0.8 ± 1.7*** | 0.023 ± 0.001*** | 0.57 ± 0.09 | 45.2 ± 8.4*** | −75.6 ± 2.3*** |
| Val434Leu | 11 | 1578 ± 145 | 204 ± 59*** | −4.1 ± 3.3 | 0.02 ± 0.002*** | 0.44 ± 0.05 | 39.6 ± 8.8*** | −83.4 ± 2.6*** |
| WT + Met430Ile | 11 | 811 ± 69* | 125 ± 23*** | 0.3 ± 1.6*** | 0.021 ± 0.001*** | 0.43 ± 0.05 | 9.1 ± 0.6*** | −81.0 ± 2.7*** |
| WT + Val432Met | 6 | 1321 ± 346 | 126 ± 20*** | 5.8 ± 1.1*** | 0.021 ± 0.001*** | 0.40 ± 0.07 | 17.1 ± 3.2*** | −63.2 ± 2.5*** |
| WT + Val434Leu | 7 | 1794 ± 319 | 233 ± 42*** | −0.4 ± 1.9*** | 0.022 ± 0.001*** | 0.38 ± 0.06 | 23.1 ± 7.7*** | −86.0 ± 2.0*** |
*p < 0.05; **p < 0.01; ***p < 0.001; vs. WT via one‐way ANOVA with post‐hoc correction via Dunn's test.