TABLE 1.
Effect of gingipain-R treatment on subsequent IL-8, MCP-1, and IP-10 production by HGF upon T-cell stimulation
| Chemokine | n | Amt of protein in culturea
|
P value | |
|---|---|---|---|---|
| Medium + HUT | Gingipain-R + HUT | |||
| IL-8 (ng/ml) | 4 | 26.7 ± 5.2 | 71.6 ± 15.4 | 0.08 |
| MCP-1 (pg/ml) | 4 | 1,455.2 ± 367 | 1,641.0 ± 479.7 | 0.3 |
| IP-10 (pg/ml) | 4 | 232.3 ± 26.6 | 149.5 ± 33.3 | 0.04 |
a
Ten thousand HGF/well were cultured for 12 h with or without gingipain-R. They were then cultured for a further 12 h with stimulated HUT-78 membranes (5 × 104 cell equivalents per condition) in 96-well plates. The levels of various chemokines were measured in the same culture supernatants by ELISA. Data are the mean ± standard error of the mean of four distinct HGF, each tested in duplicate.