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. 2022 Oct 16;20(1):25–47. doi: 10.1007/s13770-022-00487-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Rapid isolation procedure of hADSCs. A Graphical representation of the two protocols tested for hADSCs isolation. B The bar graphs represent the evaluation of cell retrieval ability divided per number of cells isolated (Cell Count), percentage of viability, percentage of adherence to the plastic and matrigel substrate, and number of hADSCs isolated (hADSCs Count). C Chondrogenic gene expression and Glycosaminoglycan assays: the bar graphs represent the fold changes calculated with 2^ΔΔCΤ method of Collagen type 2A1 (COL2A1), Aggrecan (ACAN), Sox9 (SOX9) and Collagen type 1A2 (COL1A2) markers in RT-qPCR assay. n.d. = not detectable. GAPDH was used as the housekeeping gene, and data were normalized to hADSCs at day 21. Glycosaminoglycan (GAG) content measured via the normalisation of GAG over total DNA present in the processed scaffolds. Graph bars represent standard error margin between three biological replicates. The two time points of the analysis (day 0-DO and day 21-D21) are reported in × axes of the graphs. Statistical analysis was performed using an unpaired t-test