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. 2022 Dec 7;299(1):102780. doi: 10.1016/j.jbc.2022.102780

Figure 1.

Figure 1

Coverslip-induced ischemia causes depolarization of mitochondrial inner membrane potential (ΔΨ). Shortly after the coverslip is placed over the monolayer, a wave of ΔΨ partial depolarization initiates in the center of the ischemic region and propagates toward the edge of the coverslip. Images show the TMRM signal in the dequench mode; the solid line circle shows the edge of the monolayer (22 mm diameter), and the dashed line is the edge of the ischemia-inducing coverslip (15 mm diameter).