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. 2022 Dec 7;299(1):102780. doi: 10.1016/j.jbc.2022.102780

Figure 2.

Figure 2

Dynamics of changes in [Ca2+]mduring coverslip-induced IR.A, mitoCameleon FRET ratio, normalized to average preischemic values, is shown for four neighboring cells at the center of a monolayer during IR. Cells underwent contracture between 25 to 45 min after the initiation of ischemia. Dashed lines show the beginning of ischemia and reperfusion. B, the change in mitoCameleon YFP/CFP ratio normalized to the baseline at maximum ratio during and at the end of ischemia (N = 20). C, the dynamics of concurrent changes in ΔΨ and [Ca2+]m in two cells at the center of the ischemic zone. D, the dynamics of changes in ΔΨ (TMRM intensity) and [Ca2+]m in individual mitochondrial clusters in each of the cells in (C). E, the confocal microscopy images, magnified and cropped (100 × 100 μm2) around the cell corresponding to the left panel in (C) at the beginning of the ischemic period. TMRM signal is in linear mode. Not all cells expressed the mitoCameleon Ca2+ indicator. IR, ischemia and reperfusion; TMRM, tetramethylrhodamine methyl ester.