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. 2023 Jan 19;14:330. doi: 10.1038/s41467-023-36027-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — The effect of total nsLTP concentration (a) and dissociation constant (b) on the extent of enhanced flux, and concentration gradient across the cell wall (S₀/S_L) on the extent of enhanced flux (c) and the total VOC emission flux (d). a The extent of enhanced flux was plotted against different nsLTP levels at a fixed S₀/S_L ratio and measured respective dissociation constants. b The extent of enhanced flux (black line) was plotted against different dissociation constants at a fixed S₀/S_L ratio and nsLTP level (1 mM). The vertical red line indicates the predicted optimal dissociation constant, and the six vertical dotted lines show the experimentally obtained in the TNS-binding assay dissociation constants of PhnsLTP3 with benzylbenzoate (orange), vanillin (light blue), benzyl alcohol (yellow), methylbenzoate (green), benzaldehyde (pink), and 2-phenylethanol (purple) from left to right, respectively. c The extent of enhanced flux was plotted against different S₀/S_L ratios at a fixed nsLTP level (1 mM) and measured respective dissociation constants. d The total emission flux was plotted against different S₀/S_L ratios assuming nsLTP levels in wild-type 1 mM (blue), in PhnsLTP3-RNAi flowers 0.6 mM (orange), and no nsLTP (green). Abbreviations: S₀ and S_L are VOC concentrations at the apical facing side of the plasma membrane and the inner side of the cuticle, respectively (see Supplementary Fig. 18). The extent of enhanced flux (E.o.F) in a–c and emission flux (J) in d were calculated based on Eq. 10 and Eq. 9, respectively (see Supplementary Methods). The values of the parameters used are provided in Supplementary Table 6.