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. 2022 Dec 23;28:88–103. doi: 10.1016/j.omto.2022.12.005

Figure 3.

Figure 3

BBOX1-AS1 regulates autophagy and HCC cells sensitivity to sorafenib

(A) Western blot was performed to detect the levels of autophagy-related proteins in HCC cells after transfection. (B) CCK-8 assays were performed to explore the role of BBOX1-AS1 in regulating sorafenib sensitivity in HCC (sorafenib: 10 μM). (C) Confocal microscopy was used to monitor the autophagosomes labeled by RFP-GFP-tagged LC3. Red dots indicate autolysosomes; yellow dots indicate autophagosomes (sorafenib: 10 μM). (D) TEM images were used to observe autophagosomes (sorafenib: 10 μM). (E) Western blot was performed to detect the role of BBOX1-AS1 and sorafenib in regulating autophagy (sorafenib: 10 μM). (F) Apoptosis assays were performed to explore the role of BBOX1-AS1 and sorafenib in cell apoptosis (sorafenib: 10 μM). (G) CCK-8 assays were performed to detect that 3-mA (2 mM) and rapamycin (10 nM) reverse the effect of sorafenib sensitivity induced by BBOX1-AS1 overexpression or knockdown. #p < 0.05, ##p < 0.01, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.