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. 2022 Dec 23;28:88–103. doi: 10.1016/j.omto.2022.12.005

Figure 4.

Figure 4

BBOX1-AS1 regulates autophagy and promotes HCC progression via regulation of PHF8

(A) qRT-PCR analysis of miR-361-3p expression in HCC tissues and matched adjacent normal tissues (n = 83). (B) Correlation analysis showing a negative correlation between miR-361-3p and BBOX1-AS1 expression. (C) qRT-PCR analysis of miR-361-3p expression in BBOX1-AS1 overexpression and silencing of HCC cells. (D) Schematic diagram of the binding sites between BBOX1-AS1 and miR-361-3p. (E) Dual-luciferase reporter assays analyzing the binding between BBOX1-AS1 and miR-361-3p. (F) RIP assays verified the binding between BBOX1-AS1 and miR-361-3p. (G) qRT-PCR analysis of PHF8 expression in HCC tissues and matched adjacent normal tissues (n = 83). (H) Correlation analysis showing a positive correlation between BBOX1-AS1 and PHF8 expression. (I) Western blot analysis of PHF8 expression in HCC tissues and matched adjacent normal tissues. (J and K) correlation between the PHF8 expression and miR-361-3p was analyzed in HCC cells by qPCR and WB. (L) Schematic diagram of the binding sites between miR-361-3p and PHF8-3′UTR. (M) Dual-luciferase reporter assays analyzing the binding between miR-361-3p and PHF8-3′UTR. (N) RIP assays verified the binding between miR-361-3p and PHF8 mRNA. ∗∗p < 0.01, ∗∗∗p < 0.001; ns, no significance. N, normal tissue; T, tumor tissue.