Figure 5.

PHF8 is involved in BBOX1-AS1-modulated HCC progression and sorafenib resistance

(A) Western blot was performed to detect that BBOX1-AS1 can regulate PHF8 expression level in HCC cells. (B and C) CCK8 and colony formation assays were performed to explore the ability of proliferation in HCC cells with indicated transfection. (D) Cell metastasis ability in transfected HCC cells was analyzed by Transwell assays. (E) Confocal microscopy was used to monitor the autophagosomes labeled by RFP-GFP-tagged LC3. Red dots indicate autolysosomes; yellow dots indicate autophagosomes (sorafenib: 10 μM). (F) Drug sensitivity assays were performed in HCC cells with indicated transfection. (G) EMT and autophagy-related proteins were detected by western blot. ##p < 0.01, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.