Figure 6.

BBOX1-AS1 promotes HCC progression and sorafenib resistance through the miR-361-3p/PHF8 axis

(A) Western blot was performed to detect that BBOX1-AS1 can regulate PHF8 expression level through the miR-361-3p/PHF8 axis. (B–D) CCK8, colony formation, and Transwell assays were performed to demonstrate that BBOX1-AS1 can promote cell proliferation and metastasis ability of HCC cells through the miR-361-3p/PHF8 axis. (E and F) Confocal microscopy images and drug sensitivity assays showed that BBOX1-AS1 can promote autophagy and sorafenib resistance of HCC cells through the miR-361-3p/PHF8 axis. (G) Western blot was performed to demonstrate that BBOX1-AS1 could modulate EMT and autophagy process through regulating miR-361-3p/PHF8. ##p < 0.01, ###p < 0.001, ∗∗p < 0.01, ∗∗∗p < 0.001.