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. 2023 Jan 19;14:323. doi: 10.1038/s41467-023-35976-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a–h N. benthamiana leaf protoplasts ectopically expressing different remorins from the non-symbiotic plant Arabidopsis thaliana covering all sub-groups of the Arabidopsis remorin family. AtREM1.3 (a, b), AtREM1.4 (c), AtREM2.1 (d), AtREM3.2 (e), AtREM4.2 (f), AtREM5.1 (g), AtREM6.1 (h). i–k Membrane tubulation activity was also assessed for a N-terminally truncated variant of AtREM1.3 (AtREM1.3^Cterm, i), a chimeric protein comprised of the AtREM1.3 N-terminal and the SYMREM1 C-terminal region (AtREM1.3^Nterm::SYMREM1^Cterm, j), a dual-legume SYMREM1 chimera (LjSYMREM1^Nterm::SYMREM1^Cterm, k) and SYMREM1 (l). Scale bars indicate 10 μm. All images are shown as maximal projections. The magenta signal indicates chlorophyll autofluorescence from chloroplasts. Numbers indicate the frequencies of the presented observations across at least three independent biological replicates.