TABLE 2.

Levels of IgG1 and IgG2a antibodies to purified mutant LF and PA proteins

Treatment	Anti-PA		Anti-LF
	IgG1	IgG2a	IgG1	IgG2a
PAa	0.6	0.5
LFb			38	0.2
LF and PAc	0.3	0.1	69	0.1
PA prime boostd	2	0.1
LF prime booste			1,164	2.7
PA and LF prime boostf	13	4	538	2.5

^aSera collected from mice immunized with a DNA vaccine plasmid encoding PA. 

^bSera collected from mice immunized with a DNA vaccine plasmid encoding LF. 

^cSera collected from mice immunized with a DNA vaccine plasmid encoding PA and LF. 

^dSera collected from mice immunized with a DNA vaccine plasmid encoding PA and boosted with 12.5 μg of purified PA protein. 

^eSera collected from mice immunized with a DNA vaccine plasmid encoding LF and boosted with 12.5 μg of purified LF protein. 

^fSera collected from mice immunized with a DNA vaccine plasmid encoding PA and LF and boosted with 12.5 μg of purified PA and LF protein.