Table 1.
Overview of used primers, methods and results of genetically species identification including literature sources. Species marked with * were additionally confirmed with morphological methods.
| Order | Accession number | Species | Primer | Thermocycling | Query Length | Query Cover | Per. Ident. | Sources |
|---|---|---|---|---|---|---|---|---|
| Nematoda | SAMN32641565 | Porrocaecum ensicaudatum* | forward primer rDNA2 (5′-TTGATTACGTCCCTGCCCTTT-3′) reverse primer rDNA1. 5.8s (5′-ACGAGCCCGAGTGATCCACCG-3′) | 120s at 94 °C, 40 x (60s at 94 °C, 60s at 57 °C, 120s at 72 °C) | 603 | 77% | 98.51 |
Powers et al. (1997) Morphology: Anderson et al. (2009), Osche (1959) |
| SAMN32641562 | Baylisascaris procyonis | forward primer 988F (5′-CTCAAAGATTAAGCCATGC-3′) reverse primer 1912R (5′-TTTACGGTCAGAACTAGGG-3′) | 94 °C for 5 min; 5 x (94 °C, 30 s; 45 °C, 30 s; 72 °C, 70 s) 35 x (94 °C, 30 s; 54 °C, 30 s; 72 °C, 70 s) 300s at 72 °C | 934 | 99 | 100 | Holterman et al. (2006) | |
| SAMN32641568 | Baylisascaris procyonis | forward primer 28S-rD1.2a (5′- CCCSSGTAATTTAAGCATATTA-3′) reverse primer 28SB (5′-TCGGAAGGAACCAGCTACTA-3′) | 180s at 95 °C, 40 x (30s at 95 °C, 30s at 54 °C, 60s at 72 °C) 600s at 72 °C | 1067 | 99 | 99.81 | Whiting (2002) | |
| Digenea | SAMN32641558 | Brachylaima mesostoma* | forward primer dig12 (5′- AAGCATATCACTAAGCGG-3′) reverse primer 1500R (5′- GCTATCCTGAGGGAAACTTCG-3′) | 98 °C for 10 s, 40x (50 °C for 20 s, 68 °C for 90 s, 60s at 72 °C) | 1096 | 99 | 99 |
Gérard et al. (2020) Morphology: Heneberg et al. (2016) |
| SAMN32641566 | Euryhelmis squamula* | forward primer WormA (5′-GCGAATGGCTCATTAAATCAG-3′) reverse primer WormB (5′-CTTGTTACGACTTTTACTTCC-3′) | 180s at 94 °C; 40x (30s at 94 °C, 30s at 56 °C, 120s at 72 °C); 420s at 72 °C | 1145 | 100 | 99.56 |
Nugaraitė et al. (2017), Littlewood and Olson (2001) Morphology: Anderson and Pratt (1965) |
|
| SAMN32641560 | Alaria alata | 1239 | 99 | 97.68 | ||||
| Acanthocephala | SAMN32641567 | Polymorphus minutus | forward primer LCO1490 (5′-GGTCAACAAATCATAAAGATATTGG-3′) reverse primer HCO 2198 (5′-ACTAAAAAACCAGTGGGACTTCAAAT-3′) | 120s at 95 °C; 35x (60s at 95 °C, 60s at 40 °C, 90s at 72 °C); 420s at 72 °C | 608 | 100 | 93.09 | Folmer et al. (1994) |
| Arachnida | SAMN32641559 | Ixodes hexagonus | 641 | 99 | 99.58 | |||
| SAMN32641569 | Ixodes ricinus | 649 | 100 | 99.08 | ||||
| Insecta | SAMN32641561 | Chaetopsylla globiceps | forward primer F-Leu (5′-TCT AAT ATG GCA GAT TAG TGC-3′) reverse primer R-Lys (5′- GAG ACC AGT ACT TGC TTT CAG TCA TC- 3′) | 300s at 95 °C; 40x (40s at 94 °C, 60s at 53 °C, 60s at 72 °C); 420s at 72 °C | 726 | 95 | 99.42 |
Hornok et al. (2018) Morphology: Brinck-Lindroth and Smit (2007) |
| SAMN32641564 | Ctenocephalides felis | 711 | 99 | 99.58 | ||||
| SAMN32641563 | Paraceras melis* | forward primer 18Sa1.0 (5′-GGT GAA ATT CTT GGA YCG TC-3′) reverse primer 18S9R (5′-GAT CCT TCC GCA GGT TCA CCT AC-3′) | 300s at 95 °C; 40x (40s at 94 °C, 60s at 55 °C, 60s at 72 °C); 420s at 72 °C | 839 | 100 | 98.21 |