FIG. 1.

Activation of DC following infection with P. aeruginosa. DC were incubated with PAO1 at 10 CFU/cell for 2 h. The DC were then incubated with gentamicin to kill live bacteria. After 48 h, the DC were stained with anti-B7.2–FITC (CD86) and anti-B7.1–phycoerythrin (CD80). Shown are results of flow cytometry of naive DC (A) and PAO1-pulsed DC (B). The gates were set at <1% positive cells for DC stained with an FITC-labeled isotype control IgG.