FIG. 4.

Immunization with P. aeruginosa-pulsed DC partially protects against subsequent infection with a lethal dose of P. aeruginosa pulmonary infection. DC were incubated with live (●) or heat-inactivated (□) PAO1 for 3 h, washed, and then readministered intravenously to syngeneic mice. Naive mice (○) and mice receiving naive DC (▪), LPS-stimulated DC (▵), or DC pulsed with E. coli (▴) served as controls. Two weeks later, PAO1 encapsulated in agarose beads (2 × 10⁴ CFU) was administered to the animals intratracheally and survival was evaluated over time (n = 15 animals/group for animals who received PAO1-pulsed or naive DC, n = 5 animals/group for animals who received LPS-stimulated or E. coli-pulsed DC).