TABLE 3.
Recovery of B. abortus S19 and B. abortus S19 cgs mutant from the spleens of BALB/c micea
| Dose (CFU) | Time (wks) | Log CFU/spleen inoculated with:
|
Wt (mg) of spleen inoculated with:
|
||||
|---|---|---|---|---|---|---|---|
| S19 | BAI129 | BAI129(pBA19) | S19 | BAI129 | BAI129(pBA19) | ||
| 7 × 108 | 2 | 7.04 ± 0.04 | 5.56 ± 0.19 | 7.11 ± 0.08 | 993 ± 21 | 668 ± 13 | 1,140 ± 44 |
| 4 | 5.98 ± 0.06 | 3.19 ± 0.01 | 5.00 ± 0.07 | 409 ± 13 | 189 ± 8 | 392 ± 32 | |
| 5 × 106 | 2 | 6.88 ± 0.05 | 5.70 ± 0.09 | NDb | 750 ± 33 | 131 ± 7 | ND |
| 4 | 6.98 ± 0.04 | 1.70 ± 0.01 | ND | 564 ± 12 | 104 ± 7 | ND | |
| 103 | 2 | 6.2 ± 0.02 | 1.9 ± 0.02 | ND | 102 ± 11 | 90 ± 10 | ND |
| 4 | 5.1 ± 0.05 | 0 | ND | 115 ± 12 | 87 ± 9 | ND | |
a
Mice inoculated with 7 × 108 CFU of B. abortus cgs mutant complemented with plasmids pBA19 or pCD523 showed values not statistically different from those observed with the wild-type parental strain. Mice were inoculated intraperitoneally with different doses of B. abortus S19 or B. abortus BAI129 cgs mutant. At different times postinfection, five mice were killed and their spleens were processed as described in Materials and Methods. The mean spleen weight for noninfected mice was 90 ± 10 mg. Bacterial doses were calculated retrospectively. Results are means ± standard errors of values obtained in triplicate.
b
ND, not determined.