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. 2023 Jan 6;13:1069207. doi: 10.3389/fimmu.2022.1069207

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Copyright © 2023 Longo, Aloi, Lo Presti, Fiannaca, Longo, Adamo, Urso, Meraviglia, Bongiovanni, Cibella and Colombo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immuno-characterization of M(0) THP-1 macrophage-like treated with sEVs^PBDE+LPS Macrophage sEVs^PBDE+LPS on resting M(0) THP-1 macrophage-like affecting expression of surface markers of macrophage polarization by flow cytometry analysis. (A) gating strategy analyzing the selected macrophage population for CD14/CD11b expression in each experimental condition. (B) Cumulative analysis of resting M(0) THP-1 macrophage-like treated with macrophage sEVs^PBDE+LPS, or sEVs^PBDE+LPS, indicated percentage of the CD14/CD11b cells expressing CCR7, CD80 and HLA-DR. Untreated cells (NT) and LPS were considered as experimental control condition. (C) Cumulative analysis of the Mean Fluorescence Intensity (MFI) of resting M(0) THP-1 macrophage-like treated with macrophage sEVs^PBDE+LPS expressing CCR7, CD80 and HLA-DR. (D) Cumulative analysis of resting M(0) THP-1 macrophage-like treated with macrophage sEVs^PBDE+LPS, or only DMSO, indicated percentage of the CD14/CD11b cells expressing CD38 and CD209. Untreated cells (NT) and LPS were considered as experimental control condition. Non statistical significance is indicated as ns. *P ≤ 0.05 by nonparametric Mann-Whitney test, unpaired and one-tailed with confidential interval 95%.