TABLE 2.

Fluorescence parameters of HA-pHLIPs in state I (HA-pHLIP in solution at pH8), state II (HA-pHLIP with POPC liposomes in solution at pH8) and state III (Ha-pHLIP with POPC liposomes in solution at pH3), and the mid-points (pK) and cooperativities (n) of state II to state III transitions are shown.

HA-pHLIP agent	λmax in state I, II and II, nm	State II to state III transition
HA-pHLIP1	352, 350, 339	pK = 5.5, n = 2.6
HA-Peg12-pHLIP2	352, 350, 340	pK = 5.1, n = 1.8
HA-Peg12-pHLIP4, v1	347, 343, 337	pK = 6.4, n = 1.7
HA-Peg12-pHLIP4, v2	348, 343, 337	pK = 6.8, n = 2.2
2(HA-Peg12)-pHLIP3	351, 351, 337	pK = 4.8, n = 1.9
2(3xHA-Peg12)-pHLIP3
2(HA-Peg12)-pHLIP4	347, 344, 337	pK = 5.8, n = 1.3
2(HA-Peg12)-pHLIP5	347, 346, 340	pK = 6.2, n = 0.9
2(HA-Peg24)-pHLIP3	351, 350, 337	pK = 4.6, n = 0.9
2(HA-Peg24)-pHLIP4	347, 347, 336	pK = 5.8, n = 0.7