TABLE 3.
Results of coincubation assays of the isolated vesicles with immune cells a .
| Assay | MTEX | Non-MTEX | HD sEV |
|---|---|---|---|
| % T-cell with CD69 expression (flow cytometry) | 45% (p = 0.0005) | 90% | 90% |
| CD8+T-cell NF-κB activation (p65 nuclear trans-location by confocal microscopy | Yes | No | No |
| %CD8+T-cell Proliferation (CSFE) | 25% (p = 0.0005) | 85% | 75% |
| %CD8+T-cell Apoptosis (Annexin/PI binding) | 83% (p = 0.0005) | 20% | 25% |
| %NK cells with surface NKG2D expression | 70% (p = 0.001) | 95% | 98% |
| CD8+T-cell Changes in CD69 transcripts (RT-PCR) | Decrease | No change | No change |
a
Primary human T cells and NK cells were co-incubated with paired MTEX, and non-MTEX, or with sEV from HDs (6 h for apoptosis, NKG2D assays or RT-PCR; 72 h for CSFE assays and 30min for NF-κB activation). The data are median values of results obtained in experiments performed in triplicates for 12 patients with metastatic melanoma (Sharma et al., 2020).