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. 2023 Jan 17;11(1):e005319. doi: 10.1136/jitc-2022-005319

Figure 1.

Figure 1

Tasquinimod inhibits MM cell proliferation and reduces colony formation in vitro. (A) Apoptosis was analyzed by flow cytometry using Annexin V/7-AAD staining of tasquinimod-treated MM cell lines including LP-1, OPM-2, RPMI-8226 and 5TGM1 at indicated concentrations for 24 and 48 hours (n=3). (B) Cell proliferation of tasquinimod-treated MM cells (10, 25 µM) was investigated using BrdU staining at 24 hours and 48 hours. Various human MM cell lines were tested including LP-1 (n=3), OPM-2 (n=3), RPMI-8226 (n=4) and 5TGM1 (n=5). (C) Apoptosis and cell proliferation of the human stromal cell line HS-5 treated/untreated with tasquinimod (10, 25 µM) was detected by Annexin V/7-AAD (n=3) and BrdU staining (n=4). (D) Methylcellulose colony formation assays were used for LP-1 and 5TGM1 cell lines treated with vehicle or tasquinimod (10, 25 µM) for 14 days. Quantification of colony numbers was also shown (n=4). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, Mann-Whitey U test, Error bars indicate SD. 7-AAD, 7-aminoactinomycin D; MM, multiple myeloma; ns, not significant.