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. 2023 Jan 19;11(1):e005902. doi: 10.1136/jitc-2022-005902

Figure 2.

Figure 2

Mucosal-associated invariant T (MAIT) cells were enriched in tumor tissues potentially via the CCR6-CCL20 axis in non-small cell lung cancer (NSCLC) patients. (A) Scheme of the study design. Flow cytometry was applied to analyze MAIT cells distribution in tumor and paratumor tissues of patients with NSCLC. (B, C) Representative plots of MAIT cells (gated on CD3+ CD161+ TCR Vα7.2+) in tumor and paratumor tissues of NSCLC patients and its summary data (paratumor, n=31; tumor, n=37). (D) Representative plots of Ki-67 expression in MAIT cells from tumor and paratumor tissues of NSCLC patients and its summary data (n=14). (E) Representative Annexin V/7-ADD dot plots that demonstrate the percentage of apoptotic MAIT cells in tumor and para-tumor tissues of NSCLC patients and its summary data (n=12). (F) Relative expressions of chemokines that recruit MAIT cells in tumor and para-tumor tissues of NSCLC patients (n=20) using quantitative real-time PCR (RT-qPCR). (G) Expression of CCL20 in paired tumor and paratumor tissues of NSCLC patients (n=30) by ELISA. (H, I) Tumor and paratumor tissue sections were stained with hematoxylin and eosin (HE) (left) and immunofluorescence staining for CD3, TCR Vα7.2, and CCL20 (right) and its summary data. Immunofluorescence was performed on paired paratumor and tumor tissues from 7 NSCLC patients. Each dot represents one individual high-power field. Scale bar, 100 µm or 50 µm. The upper and lower ends of the boxes represented IQR of values. The lines in the boxes represented median value. Statistical significance was calculated via Mann-Whitney U test.