Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 19;11(1):e005902. doi: 10.1136/jitc-2022-005902

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

The chemotaxis and functional phenotype of mucosal-associated invariant T (MAIT) cells from PBMCs in non-small cell lung cancer (NSCLC) patients. (A) Scheme of the study design. Flow cytometry was applied to analyze MAIT cells from PBMCs of healthy donors (HD) and NSCLC patients. (B and C) Representative plots of MAIT cells (gated on CD3⁺CD161⁺TCR Vα7.2⁺) in peripheral blood of healthy donors and NSCLC patients and its summary data. (HD, n=16; NSCLC, n=61). (D) Flow chart of in vitro MAIT cell chemotactic migration assay. The purity of isolated MAITs is shown. (E) Summary data of MAIT cell migration treated with or without the neutralizing antibody (±anti-CCL20) in the bottom. MAIT cells (10³) induced in each condition were evaluated (n=6). (F) Representative plots of MAIT cell subsets (gated on CD3⁺ CD161⁺ TCR Vα7.2⁺) from PBMCs of healthy donors (HD) and NSCLC patients. (G) MAIT cell subset composition in peripheral blood of HD and NSCLC patients. (H) Quantification of CD8⁺ MAIT cells and CD4⁺ MAIT cells in CD3⁺ T cells in peripheral blood of HD and NSCLC patients. (HD, n=14; NSCLC, n=22). (I and J) Expression of the activation markers HLA-DR and CD38 on MAIT cells from PBMCs of HD and NSCLC patients detected by FCM (gated on CD3⁺ CD161⁺ TCR Vα7.2⁺) and their summary data. (HD, n=16; NSCLC, n=61). (K and L) Expression of the immune inhibitory molecules PD-1 and TIM-3 on MAIT cells from PBMCs of HD and NSCLC patients detected by FCM (gated on CD3⁺ CD161⁺ TCR Vα7.2⁺) and their summary data. (HD, n=16; NSCLC, n=61). (M and N) Expression of the effector molecules IFN-γ and IL-17A in MAIT cells from PBMCs of HD and NSCLC patients detected by FCM (gated on CD3⁺ CD161⁺ TCR Vα7.2⁺) after stimulated with PMA and ionomycin for 4 hours and their summary data. (HD, n=11; NSCLC, n=52). The upper and lower ends of the boxes represented IQR of values. The lines in the boxes represented median value. Statistical significance was calculated via Mann-Whitney U test. PBMC, peripheral blood mononuclear cells.