FIG. 3.

Flow cytometric analysis of leukocytes recruited to the brain. In each experiment, the brains of three mice per experimental group were pooled. Leukocytes were isolated by density centrifugation and counted, and the percentage of LCA^high leukocytes was determined by flow cytometry. The data show the mean number of LCA^high leukocytes per brain and the standard deviation of two independent experiments. LCA^low brain resident microglial cells were not included. The asterisks indicate that IL-10^−/− mice had significantly increased numbers of inflammatory leukocytes compared to the respective group of WT mice; the dagger indicate deceased animals; nd, not done.