Fig. 3.
Analog 8 inhibits N-type (Cav2.2) calcium currents in dorsal root ganglia neurons. (A) Representative family of N-type calcium currents evoked by voltage steps from −70 to +60 mV for 200-milliseconds. (B) Current-voltage relationship of control (0.1 %-DMSO), compound 8, evoked from −70 to +60 mV for 200-milliseconds. (C) Peak calcium current density summarized as scatter plots (DMSO −44.0 ± 7.52 (n = 11), 8 –18.8 ± 2.7 (n = 10)). (D) Steady-state inactivation and activation curves. To evaluate steady-state inactivation, currents were elicited by a 20 ms pulse at 10 mV after a 1500 ms pre-pulses ranging from −100 mV to 30 mV in 10 mV increments from a holding potential of −60 mV. Table 2 summarizes the half-maximal activation potential of activation and inactivation (V1/2) and slop values (k) for voltage-dependence activation and inactivation. n = 10–11 cells; error bars indicate mean ± SEM; p values as indicated (see Table S1 for full statistics).