Fig. 4.

T-type calcium currents are reduced by compound 8. (A) Representative T-type Ca²⁺ current traces evoked by voltage steps from −70 to 0 mV in increments of 5 mV. (B) Average current density–voltage plot of control (0.1 % DMSO) and compound 8 (20 µM). Currents were measured from a holding potential of −90 mV with voltage steps of 200 ms of duration applied at 0.5 s intervals in 5 mV increments (C) Peak current density summarized as scatter plot (0.1 %-DMSO, −60.1 ± 5.0, compound 8–35.3 ± 5.7). (D) Boltzmann fits for and steady-state inactivation curves. After a 1.5 s pre-pulses ranging from −100 mV to −10 mV in 10 mV increments from a holding potential of −90 mV. Half-maximal activation potential of activation, inactivation (V_1/2) and slope values (k) for activation and inactivation are presented in Table 3. n = 9–13 cells; error bars indicate mean ± SEM; p values as indicated (see Table S1 for full statistics).