Table 4.
Effect of a single administration (2 µg/rat i.t.) of compound 8 in ALGOGram.a
| Pain area | Model (test) | Compound 8 |
Internal reference |
|
|---|---|---|---|---|
| Percentage of analgesic activity vs vehicle | Reference ID | Percentage of activity vs vehicle | ||
| Acute and tonic pain | Healthy rats (paw pressure test) | −5.5 % | Morphine, 4 mg/kg s.c., T30 min | 67 % |
| Healthy rats (tail flick test) | 0.5 % | Morphine, 4 mg/kg s.c., T30 min | 66 % | |
| Acetic acid test (abdominal cramps) | 24.5 % | (-) U50, 488H, 4 mg/kg s.c., T30 min | 100 % | |
| Formalin test (paw licking time early phase) | 31.5 % | Morphine, 4 mg/kg s.c., T30 min | 65 % | |
| Formalin test (paw licking time late phase) | 45.5 % | Morphine, 4 mg/kg s.c., T30 min | 77 % | |
| Neuropathic pain | Bennett model (Paw pressure test) | −20 % | Morphine, 3 mg/kg s.c., T30 min | 188 % |
| Oxaliplatin (paw immersion test) | −2% | Duloxetine 100 mg/kg, p.o., T60 min |
66 % | |
| Inflammatory pain | Carrageenan (paw pressure test) | 2 % | Indomethacin 30 mg/kg, p.o., T60 min | 92 % |
| Kaolin (Gait score) | −13 % | Indomethacin 30 mg/kg, p.o., T60 min | 64 % | |
| Post-operative pain | Brennan model (Electronic Von Frey test) | 11 % | Morphine, 4 mg/kg s.c., T30 min | 107 % |
| Visceral pain | TNBS (colonic distension) | 6.5 % | (_) U50, 488H, 4 mg/kg s.c., T30 min | 168 % |
a
Testing: 120 min after treatment. n = 4/model/test. Results are expressed for each group as percentage of activity calculated from the mean value of the vehicle-treated animals and compared to naïve animals, control paw or cut-off value depending on the test (from the ANS Biotech historical database (Darbaky et al., 2017, Gris et al., 2016, Silos-Santiago et al., 2013). All experiments were done in a blinded manner.