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. 2023 Jan 6;10:1070258. doi: 10.3389/fpubh.2022.1070258

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Copyright © 2023 Alves, Castro, Pinto, Nunes, Pomba, Oliveira, Silveira, Gomes and Oleastro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogeny of Clostridioides difficile isolates from ribotypes 014/020 used in the present study. The minimum spanning tree (MST) was constructed based on the core-SNP diversity found among 183 isolates, relative to reference genome S-0352 (CP076377.1). All nodes (which represent a unique allelic profile) presenting an SNP distance ≤ 2, representing clusters with potential epidemiological relevance, have been collapsed for visualization purposes. Straight and dotted lines reflect nodes linked with the SNP distances below and above 100 respectively. Nodes are colored according to different countries of origin and their contour colored by respective source. The MST was generated using GrapeTree v1.5.0 software (33).