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Marker and locus discovery rate from genotyping by sequence after digestion of Gerbera genomic DNA with two combinations of restriction enzymes and sequencing at different read depths (number of reads per sample). The combination of BamHI and Nsi was used in the left chart and the combination of BtgI and TaqI in the right chart. Genomic DNA digestion, sequencing library preparation, and genotyping by sequencing were completed at the University of Minnesota Genomics Center.
