Figure 6.

Tamoxifen induces mitochondrial ROS production. Equine neutrophils were stained with Mitosox and incubated with tamoxifen (10 uM), rotenone (10 uM), mitotempo(200 uM) or vehicle (DMSO 0.1%) for 30 min at 37°C. Where indicated, prior to the addition of tamoxifen (10 uM) the neutrophils were preincubated with rotenone (10 uM) or mitotempo (200 uM) for 30 min at 37°C. The mean fluorescence intensity (MIF) was analyzed by flow cytometry. The result corresponds to the average +/- S.E.M of 5 independent experiments.^*p < 0.05; ^**p < 0.01.