Table 4.

Primer sets used for PCR analysis of Tenacibaculum species loci proposed in the MLST.

Locus	Primer Sequences (5′–3′)		Amplicon length (bp)	Reference
	Forward	Reverse
atpA	ATTGGWGAYCGTCAAACWGG	CCAAAYTTAGCRAAHGCTTC	567	Habib et al. (2014)
atpD	TGGYCCAGTWATCGATGTTGA	AATACGYTCTTGCATTGCTC	807	Småge et al. (2016a)
dnaK	GGWACYACNAAYTCDTGTGT	TCWATCTTMGCTTTYTCAGC	573	Habib et al. (2014)
fusA	ATGGTAACTCACCCATTCCAGA	TGGCATGATGCAACACAAGG	767	Småge et al. (2016a)
glyA	CAYTTAACWCAYGGWTCDCC	ACCATRTTTTTRTTTACHGT	558	Habib et al. (2014)
gyrB	AGTATYCARGCRCTRGAAGG	GTWCCTCCTTCRTGYGTRTT	597	Habib et al. (2014)
ileS	CCWACHTTTGGWGCHGAYGA	GAATCRAACCAWACATCAAT	542	Habib et al. (2014)
infB	ATGCCDCAAACWAAAGARGC	GTAATHGCTCCAACYCCTTT	564	Habib et al. (2014)
pgk	GCTCCWCCACCWGTAGAAAC	GCTCCWCCACCWGTAGAAAC	935	Småge et al. (2016a)
rlmN	GCKTGTGTDTCDAGYCARGT	CCRCADGCDGCATCWATRTC	549	Habib et al. (2014)
rpoB	ATYTCTCCAAAACGCTGACC	AAAACGAATCAAGGWACGAAYA	3266	Småge et al. (2016a)
	ACCCTTTCCAAGGCATAAAGG	GAGCCATYGGTTTTGAAAGAGA
	GAGCCATYGGTTTTGAAAGAGA	GAGCCATYGGTTTTGAAAGAGA
tgt	GAAACWCCWATWTTYATGCC	TAYAWYTCTTCNGCWGGTTC	486	Habib et al. (2014)
trpB	GTWGCNCGWATGAAAATGYT	CCWGGRTARTCYAATCCTGC	368	Habib et al. (2014)
tuf	AGAGAWTTATTRTCTTTCTA	GTTACCTGACCWGCWCCWAC	554	Habib et al. (2014)
	ACCTCCTTCACGGATAGC	TTACGATCGTTCGAAGCCCC	971	Småge et al. (2016a)
yqfO	GCBGAARRTTTTGAYAAYGT	AYTTCRTARGCDACYTCTTC	446	Habib et al. (2014)