TABLE 3.

Effects of SB203580 and U0126 on intracellular IL-1α, IL-1β, IL-8, IL-1ra, and TNF-α production by SAC-stimulated monocytes^a

Treatment	% of cytokine-positive CD14+-gated monocytesb
	IL-1α	IL-1β	IL-8	IL-1ra	TNF-α
RPMI	2	0	12	2	0
SAC	37	77	70	21	78
SAC + SB203580	54	89	39	15	92
SAC + U0126	27	74	74	26	81
SAC + SB203580 + U0126	18	68	71	22	87

^a Purified human monocytes (10⁶/ml) were cultured for 18 h at 37°C without or with SAC (100 μg/ml) and treated with 5 μM SB203580, with 10 μM U0126, or with a mixture of SB203580 and U0126. Intracellular cytokine production was detected using specific anti-cytokine-PE or anti-cytokine-APC MAbs. An isotypic control was used as a negative control. Monocytes were gated using either anti-CD14-FITC or anti-CD14-PE MAbs. At least 5,000 CD14⁺ events were acquired for intracellular cytokine analysis. 

^b Compared to isotypic negative controls. Results are those from one representative experiment out of three performed with cells from different donors.