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. 2023 Jan 6;145(2):1236–1246. doi: 10.1021/jacs.2c11111

Table 3. Dissociation Constants (Kd) for Binding of 14 to 20-mer Duplex, G-Quadruplex (G4), and Three-Way Junction (3WJ) DNA Measured Using Cy5.5-Labeled Oligonucleotides and FRETa.

  duplex
G4
3WJ
complex Kd (μM) r (Å) Kd (μM) r (Å) Kd (μM) r (Å)
1 2.8 ± 0.9 33.5 3.9 ± 1.3 26.8 4.3 ± 0.9 37.4
2 2.3 ± 0.5 33.1 3.8 ± 1.7 30.0 4.4 ± 0.7 35.0
3 6.3 ± 0.3 23.8 nd nd nd nd
4 2.6 ± 1.0 55.9 nd nd 6.4 ± 0.3 49.6
a

See Table 2 for precise DNA sequences used. ND = Not determined (i.e., Kd values >20 μM). Mean +/– SD of two independent experiments.