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. 2023 Jan 6;145(2):1040–1052. doi: 10.1021/jacs.2c10621

Figure 5.

Figure 5

Light-minus-dark FTIR difference spectra of unlabeled (black), 15N-amide glutamine-labeled (blue), 13C4/C10a flavin-labeled (green), and 15N-amide glutamine/13C4/C10a flavin-labeled Slr1694 (red) in the amide I spectral region. The lower panel shows the double differences of unlabeled-minus-labeled spectra of completely unlabeled minus 15N-amide glutamine-labeled difference spectra (blue) and flavin-labeled minus flavin and glutamine-labeled difference spectra (red). Both double-difference spectra show a signal at ∼1695 cm–1 that loses intensity upon glutamine labeling.