FIG. 4.

Nucleotide sequence of the putative replication region in pYVe8081. The numbers correspond to base pair positions in pYVe8081. The sequence shown is single stranded, with the deduced amino acid sequences given in single-letter amino acid code. The deduced amino acid sequence of RepB is shown above its nucleotide sequence. Possible start codons are shown in boldface, and an arrowhead following the gene name indicates the direction of transcription. An asterisk in the amino acid sequence depicts a nonsense codon. Possible Shine-Dalgarno sequences have a single line underneath them. Dots depict nucleotides that are identical in pYVe8081 and the pMU407 replicon. The possible −10 and −35 sequences for the antisense RNA (RNAI) are boxed. RNAI is underlined in boldface with an arrow that indicates the direction of transcription. Facing arrows indicate possible stem-loop structures in RNAI and repB. The double line under bp 1 to 9 denotes a possible DnaA protein-binding site. Arrows labeled R1, R2, and R3 indicate direct repeats within the AT-rich region.