TABLE 1.

Features identified in Y. enterocolitica pYVe8081

Genea	Position (bp)b	Size (amino acids)	Homologous gene by BLASTc	Functiond
oriR	1–165			Putative replication site
yopQ	c2291–2839	182	AF102990	Yop translocation control
yopT	3352–4320	322	AF102990	Yop effector
sycT	4320–4712	130	AF102990	YopT chaperone
yopM	c7723–8826	367	AF102990	Yop effector
orf 91B	8949–9167	72	AF080156	Unknown
yopD	c9653–10573	306	AF102990	Yop translocator
yopB	c10592–11797	401	AF102990	Yop translocator
sycD	c11775–12281	168	AF074612	YopB, YopD chaperone
lcrV	c12284–13258	324	AF102990	YopB, YopD secretion
lcrG	c13260–13547	95	M26405	Control of Yop release
lcrR	c13589–14029	146	AF102990	Unknown
lcrD (yscV)	c14026–16140	704	AF102990	Yop secretion
yscY	c16127–16471	114	AF102990	Yop secretion
yscX	c16468–16836	122	AF074612	Yop secretion
sycN	c16833–17204	123	AF102990	YopN chaperone
tyeA	c17191–17469	92	AF102990	Control of Yop release
yopN	c17450–18331	293	AF074612	Control of Yop release
yscN	18529–19848	439	AF074612	Yop secretion
yscO	19845–20309	154	AF074612	Yop secretion
yscP	20309–21670	453	AF074612	Yop secretion
yscQ	21667–22590	307	AF102990	Yop secretion
yscR	22587–23240	217	AF102990	Yop secretion
yscS	23242–23508	88	AF074612	Yop secretion
yscT	23505–24290	261	AF074612	Yop secretion
yscU	24290–25354	354	AF102990	Yop secretion
virG (yscW)	25930–26325	131	AF102990	YscC localization
virF	26449–27264	271	X96798	Yop regulation
yscA	27344–27442	32	AF102990	Unknown
yscB	27667–28080	137	AF102990	Unknown
yscC	28086–29909	607	AF102990	Yop secretion
yscD	29906–31162	418	AF102990	Yop secretion
yscE	31159–31362	67	AF102990	Yop secretion
yscF	31359–31622	87	AF102990	Yop secretion
yscG	31624–31971	115	AF102990	Yop secretion
yscH	31968–32465	165	AF102990	Unknown
yscI	32466–32813	115	AF102990	Yop secretion
yscJ	32820–33554	244	AF102990	Yop secretion
yscK	33554–34183	209	AF102990	Yop secretion
yscL	34129–34800	223	AF102990	Yop secretion
yscM1	35019–35366	115	AF102990	Yop regulation
yopH	36048–37454	468	AF102990	Yop effector
sycH	c37676–38107	143	AF102990	YopH chaperone
sycE	c38461–38853	130	M34278	YopE chaperone
yopE	39046–39705	219	M34278	Yop effector
orf76	40996–41292	98	AF074612	Unknown
orf75	c41131–41433	100	AF074612	Unknown
orf80	c41426–41668	80	AF056093	Unknown
spyC	c42557–42781		AF074612	pYV partition site
spyB	c42930–43892	320	AF102990	pYV partition protein
spyA	c43892–45058	388	AF102990	pYV partition protein
orf73	c45372–45587	71	T00231	Unknown
yomA	c46108–46983	291	AF056092	Unknown
yadA	c47053–48321	422	X13881	Adhesin
ISYen1	49547–50841		AF074611	Unknown
orf78	c50936–51244	102	No database match	Unknown
orf79	c51244–51873	209	AF302424	Unknown
orf81	c52048–52782	244	AP001918	Putative recombinase
orf83	c52919–53116	65	No database match	Unknown
orf86	c54868–55134	88	AF264951	Unknown
orf155	55296–55727	143	AF074612	Putative Yop chaperone
yopO	55745–57934	729	AF102990	Yop effector
yopP	58301–59167	288	AF102990	Yop effector
yscM2	59786–60136	116	AF102990	Yop regulation
orf106	c61616–62167	183	AF074611	Unknown
IS1541C	65189–65896		AJ238015	Unknown
repC	65992–66237	81	U27345	Putative replication protein
RNAI	c66399–66481		U27345	Putative ctRNA
repB	66499–66555	18	U27345	Putative replication protein
repA	66548–67570	340	U27345	Putative replication protein

^aY. enterocolitica terminology is used for the LCR stimulon genes; alternate gene designations are in parentheses. Intact IS elements and putative replication-partition features are included. 

^b“c” denotes a gene encoded on the minus strand. 

^cThe accession number of the GenBank match with the highest probability value was used. In cases where the match with the highest probability value was found in more than one Yersinia spp., the Y. enterocolitica pYVe227 sequence was used. 

^dFunctions of LCR stimulon genes are from a review by Cornelis et al. (6).