TABLE 2.
Putative genes of unknown function in pYVe8081
| Gene | Position (bp)a | Shine-Dalgarno sequence and start codon | Homologous protein by BLAST (target) | % Homologyb | Accession no. (reference) |
|---|---|---|---|---|---|
| orf91B | 8949–9167 | AGAAGN4ATG | Unknown protein from pYVe227 | 91/75 | AF080156 (21) |
| orf76 | 40996–41292 | AGGACGN4ATG | Unknown protein from pCD1 | 93/100 | AF074612 (39) |
| orf75 | c41131–41433 | GGTGN10GTG | Unknown protein from pCD1 | 94/100 | AF074612 (39) |
| orf80 | c41426–41668 | GGAGGN4ATG | Unknown protein from pYVe227 | 100/100 | AF056093 (21) |
| orf73 | c45372–45587 | AGGAGN6ATG | No database match | ||
| yomA | c46108–46983 | GGTGN10ATG | Unknown protein (YomA) from pYVe227 | 97/100 | AF056092 (21) |
| orf78 | c50936–51244 | GGAGGN3ATG | No database match | ||
| orf79 | c51244–51873 | AGGTGN5ATG | Partition protein from Actinobacillus actinomycetemcomitans | 26/99 | AF302424 |
| orf81 | c52048–52782 | GCGGAN7ATG | Possible site-specific recombinase on the F plasmid | 50/99 | AP001918 |
| orf83 | c52919–53116 | GGAGCN10ATG | No database match | ||
| orf86 | c54868–55134 | AGGAGGN5ATG | Unknown protein from Erwinia amylovora | 76/55 | AF264951 (28) |
| orf155 | 55296–55727 | AGAGN7GTG | Possible chaperone from pCD1 | 95/100 | AF074612 (39) |
| orf106 | c61616–62167 | GGCAN2ATG | Possible endonuclease from Y. pestis pMT1 | 43/76 | AF074611 (24) |
a
“c” denotes a gene encoded on the minus strand.
b
Percentage of identical amino acids compared with the percentage of the total target protein sequence.