Fig. 3.

Elevation of BMP2 contributes to the aggravated valvular calcification in the context of myeloid CCN3 deficiency in vivo. A Immunofluorescence of BMP2 and CD31 on calcified murine aortic valves. (BMP2: red; CD31: green; DAPI, blue; scale bar: 100 μm, 200 μm). C Immunofluorescence of BMP2 and Vimentin on calcified aortic valves. (BMP2: red; Vimentin: green; DAPI, blue; scale bar: 100 μm). E Immunofluorescence of BMP2 and CD68 on calcified aortic valves. (BMP2: red; CD68: green; DAPI, blue; scale bar: 100 μm). B, D and F Quantification of percentage of co-stained area of red and green signal on calcified murine aortic valves (n = 5). All Statistical analysis were two-way ANOVA followed by Sidak multiple comparison test. N = biological replicates. Control: Lysm^Cre/Cre mice; Mye-CCN3-KO: Myeloid specific CCN3 knock out mice.**p < 0.01