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. 2023 Jan 20;13:13. doi: 10.1186/s13578-023-00959-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Unc5b suppresses migration of macrophage foam cells Raw 264.7 cells were subjected to various concentrations of ox-LDL (0, 24, 50 and 75 μg/ml) for 24 h, F-actin was stained with phalloidin-iFluor 488 (green), and the nuclei were counterstained with DAPI (blue), pseudopodia of macrophages were marked in white arrows (A), the number of cells on the dark side was identified by Transwell assay (B). (C) Western blot analysis of Unc5b protein levels in Raw 264.7 cells with Unc5b^oe or Unc5b^siRNA. Quantification of Unc5b expression from Western blotting. Data are reported as the mean ± S.D. (n＞3, * p< 0.05vs vehicle group, ^##p< 0.01 vs Unc5b^oe group, t-test). (D) Raw 264.7 macrophages were pretreated with Unc5bsiRNA or pcDNA3.1-Unc5b plasmid combined with ox-LDL for another 24 h, cells were stained by F-actin (green), DAPI (blue), and pseudopodia (marked in white). (E, F) Raw 264.7 cells were pretreated with empty plasmid or Unc5b^siRNA or Unc5b^oe, the number of cells on the dark side was identified by Transwell assay upon ox-LDL (50 μg/ml) treatment. Data are reported as the mean ± S.D. (bar=100 μm, n=4, ^***, p< 0.001 vs ox-LDL 0 μg/mL group,^###, p< 0.001 vs ox-LDL 50 μg/mL, t-test)