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. Author manuscript; available in PMC: 2023 Jan 20.
Published in final edited form as: Nat Neurosci. 2022 Jun 20;25(7):967–974. doi: 10.1038/s41593-022-01094-6

Extended Data Fig. 8 ∣. HcKCR1 expression in mouse cortical neurons.

Extended Data Fig. 8 ∣

a, Fluorescence images showing HcKCR1-EYFP (green) and tdTomato (magenta) expression in layer 2/3 pyramidal neurons. HcKCR1-EYFP is expressed at high levels and forms some intracellular aggregates, as do many other wild-type ChRs. HcKCR2-EYFP shows the same degree of aggregation. Membrane targeting of both KCRs is confirmed by robust photocurrents (Fig. 5b and Extended Data Fig 9.). b, The fluorescence image of HcKCR1-EYFP from Fig. 5a (left panel) was overexposed to visualize the presence of HcKCR1-EYFP in the dendrites and axons (right panel). Note, the axons of layer 2/3 pyramidal neurons ramify in layer 5. Similar results were observed in 14 slices from 2 male and 2 female mice at the age of 3–4 weeks.