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. Author manuscript; available in PMC: 2023 Jan 20.
Published in final edited form as: Optica. 2019 May 23;6(6):709–715. doi: 10.1364/optica.6.000709

Fig. 3.

Fig. 3.

(a) Normalized emission spectra of AF647 (red) and CF660C (green) with their centroids (686 nm for AF647 and 695 nm for CF660C), respectively. (b) The histogram of spectral centroid of multi-color 3D sSMLM image of tubulin and TOM20, labeled with AF647 and CF660C, respectively. To separate the two dyes, we defined two different color channels: (1) 674 to 689 nm for AF647 and (2) 692 nm to 707 nm for CF660C.