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Fluorescence microscopic analyses of HeLa cells infected with serovar D′, D, F, E, and L2 (panels A to E, respectively) and noninfected cells (F) fixed for microscopy 20 to 30 h postinfection. Immunostaining with anti-IncA indicates incA expression and the occurrence of fusogenicity in all infected cells, despite the presence of the mutations I47T and E116K (A and B), the mutation I47T (C and D), or any other polymorphisms in incA (E). The bar in each panel indicates 10 μm.
