Skip to main content
. 2023 Jan 3;12(1):114. doi: 10.3390/antiox12010114

Figure 1.

Figure 1

The effect of juglone treatment on the quality and developmental health of bovine oocytes. Oocytes were treated with 25 µM of juglone during in vitro maturation (IVM), four replicates (n = 20), and three replicates (n = 50) were used for determining the filamentous actin (F-actin) and RT-qPCR experiments, respectively. (A) Phalloidin-based staining explored the distribution of F-actin in oocytes. (B) Fluorescence intensity of stained F-actin in treated and untreated oocytes. (C,D) Relative expression of oocyte quality- and development- related genes. (C) The mRNA levels of P21, P27/GSK-3A, GSK-3B/ GDF9, BMP15/MFN1, MFN2 regulating cell cycle, meiosis progression, oocyte function and mitochondrial fusion, respectively. (D) The mRNA levels of histone methylation H3K27me3 (EZH1, EZH2, SUZ12, and EED), and H3K9me3 (G9a, SETDB1 and SUV39H2)-related methyltransferases. Scale bar = 100 μm. Columns with an asterisk (*) indicate statistical significance. BF: Bright field.