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. 2023 Jan 3;12(1):114. doi: 10.3390/antiox12010114

Figure 2.

Figure 2

The effect of juglone on the PI3K/AKT/mTOR pathway in oocytes. Bovine oocytes were exposed to 25 µM of juglone during in vitro maturation (IVM), three replicate (n = 50), and four replicates (n = 20) were used for RT-qPCR and immunofluorescence analysis, respectively. (A) Relative expression of PI3K, AKT, and mTOR genes using RT-qPCR. (B,D,F) Immunofluorescence of PI3K, pAKT, and pmTOR in juglone treated and untreated oocytes. (C,E,G) Fluorescence intensities of PI3K, pAKT-Ser473, and pmTOR in juglone-treated and control groups. Scale bar = 100 μm. Columns with an asterisk (*) indicate statistical significance. DAPI: 4′,6-diamidino-2-phenylindole. pAKT: phosphorylated AKT; and pmTOR: phosphorylated mTOR.