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. 2023 Jan 3;12(1):114. doi: 10.3390/antiox12010114

Figure 3.

Figure 3

Effect of juglone supplementation during IVM on autophagy. Juglone was treated at 25 µM during in vitro maturation (IVM) of bovine oocytes, four replicates (n = 20) were used for immunofluorescence analysis. (A) Immunofluorescence of Beclin-1 and LC3B in treated and untreated oocytes. (B) Fluorescence intensities of Beclin-1 and LC3B. Scale bar = 100 μm. Columns with an asterisk (*) indicate statistical significance. DAPI: 4′,6-diamidino-2-phenylindole; LC3B (MAP1LC3B): microtubule-associated protein 1 light chain 3 beta; and Beclin-1: autophagy-related gene 6.