. 2023 Jan 12;13(2):262. doi: 10.3390/ani13020262

Table 4.

Effects of NAC administration on the mRNA expression of Keap1, Nrf2, GSTO2, and HMOX1 in the liver, spleen, lung, lymph node, and gastrocnemius muscle in piglets infected with PEDV.

Items	−PEDV		+PEDV		SEM	p-Value
Items	−NAC	+NAC	−NAC	+NAC	SEM	PEDV	NAC	PEDV × NAC
Keap1
Liver	1.00 ^b	1.81 ^a	1.05 ^b	0.88 ^b	0.25	0.09	0.20	0.06
Lung	1.00 ^b	0.82 ^b	0.88 ^b	1.38 ^a	0.06	0.01	0.01	<0.001
Lymph node	1.00	0.83	1.22	1.29	0.08	<0.001	0.55	0.17
Gastrocnemius	1.00	0.99	0.88	1.14	0.07	0.81	0.11	0.11
Spleen	1.00 ^b	0.95 ^bc	0.77 ^c	1.39 ^a	0.07	0.17	0.01	<0.001
Nrf2
Liver	1.00 ^b	2.01 ^a	0.82 ^b	0.53 ^c	0.08	<0.001	<0.001	<0.001
Lung	1.00 ^c	1.55 ^a	1.29 ^ab	1.07 ^bc	0.09	0.33	0.09	<0.001
Lymph node	1.00	0.86	0.83	0.70	0.06	0.01	0.04	0.95
Gastrocnemius	1.00 ^c	1.16 ^c	3.40 ^a	1.77 ^b	0.15	<0.001	<0.001	<0.001
Spleen	1.00 ^b	0.24 ^d	0.47 ^c	1.35 ^a	0.05	<0.001	0.27	<0.001
GSTO2
Liver	1.00 ^a	0.42 ^b	0.32 ^bc	0.25 ^c	0.04	<0.001	<0.001	<0.001
Lung	1.00 ^b	1.44 ^a	1.14 ^b	1.06 ^b	0.08	0.18	0.03	0.01
Lymph node	1.00 ^a	0.21 ^bc	0.25 ^b	0.14 ^c	0.03	<0.001	<0.001	<0.001
Gastrocnemius	1.00 ^a	0.16 ^c	0.28 ^b	0.26 ^b	0.02	<0.001	<0.001	<0.001
Spleen	1.00 ^a	0.19 ^b	0.23 ^b	0.16 ^b	0.03	<0.001	<0.001	<0.001
HMOX1
Liver	1.00 ^b	1.42 ^a	0.78 ^c	0.86 ^bc	0.06	<0.001	<0.001	0.01
Lung	1.00 ^a	0.72 ^b	0.81 ^ab	0.96 ^a	0.06	0.71	0.35	0.01
Lymph node	1.00	0.73	1.75	1.15	0.09	<0.001	<0.001	0.09
Gastrocnemius	1.00	0.42	0.85	0.50	0.05	0.58	<0.001	0.06
Spleen	1.00 ^a	0.73 ^c	0.72 ^c	1.49 ^b	0.08	0.01	0.01	<0.001

Values are mean and pooled SEM, n = 8; ^a,b,c,d within a row, means with different superscripts differ, p < 0.05.