Table 5.
Effects of NAC administration on mRNA of IFN-α, MX1, OASL, and ISG15 in the liver, spleen, lung, lymph node, and gastrocnemius muscle in piglets infected with PEDV.
| Items | −PEDV | +PEDV | SEM | p-Value | ||||
|---|---|---|---|---|---|---|---|---|
| −NAC | +NAC | −NAC | +NAC | PEDV | NAC | PEDV × NAC | ||
| IFN-α | ||||||||
| Liver | 1.00 b | 1.53 a | 0.75 c | 0.54 c | 0.08 | <0.001 | 0.07 | <0.001 |
| Spleen | 1.00 a | 0.59 c | 0.80 b | 1.16 a | 0.06 | <0.001 | 0.67 | <0.001 |
| Lymph node | 1.00 d | 2.59 a | 1.66 c | 2.07 b | 0.12 | 0.55 | <0.001 | <0.001 |
| Gastrocnemius | 1.00 | 1.03 | 1.33 | 1.25 | 0.09 | 0.01 | 0.75 | 0.54 |
| MX1 | ||||||||
| Liver | 1.00 b | 1.63 a | 1.59 a | 1.56 a | 0.10 | 0.02 | 0.01 | <0.001 |
| Spleen | 1.00 a | 0.87 a | 0.51 b | 0.94 a | 0.05 | <0.001 | 0.01 | <0.001 |
| Lymph node | 1.00 | 1.55 | 1.64 | 2.24 | 0.12 | <0.001 | <0.001 | 0.83 |
| Gastrocnemius | 1.00 c | 1.28 bc | 1.80 a | 1.39 b | 0.11 | <0.001 | 0.56 | 0.01 |
| OASL | ||||||||
| Liver | 1.00 | 1.42 | 1.30 | 1.52 | 0.28 | 0.50 | 0.28 | 0.73 |
| Spleen | 1.00 a | 0.63 b | 0.60 b | 1.07 a | 0.07 | 0.78 | 0.50 | <0.001 |
| Lymph nod | 1.00 c | 0.92 c | 2.53 a | 1.74 b | 0.13 | <0.001 | <0.001 | 0.01 |
| Gastrocnemius | 1.00 b | 0.84 b | 2.27 a | 0.76 b | 0.11 | <0.001 | <0.001 | <0.001 |
| ISG15 | ||||||||
| Liver | 1.00 | 0.98 | 2.72 | 2.42 | 0.13 | <0.001 | 0.26 | 0.31 |
| Spleen | 1.00 c | 1.94 a | 1.05 c | 1.44 b | 0.10 | 0.03 | <0.001 | 0.01 |
| Lymph node | 1.00 c | 0.91 c | 1.74 b | 2.35 a | 0.11 | <0.001 | 0.03 | <0.001 |
| Gastrocnemius | 1.00 b | 1.17 b | 1.85 a | 1.30 b | 0.10 | <0.001 | 0.06 | <0.001 |
Values are mean and pooled SEM, n = 8; a,b,c,d within a row, means with different superscripts differ, p < 0.05.