FIG. 3.

TLC of acyl-HSLs. Ethyl acetate extracts were chromatographed on C₁₈ reversed-phase thin-layer plates developed with methanol-water (60:40, vol/vol). The spots were visualized using the A. tumefaciens reporter strain. Samples from each genomovar were chromatographed as follows: lane 1, B. cepacia genomovar I (strain CEP509); lane 2, B. multivorans (strain C5393); lane 3, B. cepacia genomovar III (strain K56-2); lane 4, B. stabilis (strain LMG14294); lane 5, B. vietnamiensis (strain G4); lane 6, genomovar VI (strain LMG18943); lane 7, genomovar VII (strain CEP996); lane 8, synthetic N-hexanoyl-HSL; lane 9, synthetic N-octanoyl-HSL.