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. 2001 Jul;69(7):4661–4666. doi: 10.1128/IAI.69.7.4661-4666.2001

TABLE 2.

Primers and PCR conditions for amplification of quorum-sensing genes in B. cepacia

Gene Primer Sequence Annealing temp (°C)a Amplicon size (bp)
cepI CEPI1 5′-GCGGATCC-121-ACCAGACGCCCATCTACCTGCTTCG-3′134b 59 598
CEPI2 6995′-GTTACCAGTTACAGGCTCCTC-3′679
cepI CEPI1 5′-GCGGATCC-121-ACCAGACGCCCATCTACCTGCTTCG-3′134 59 278
CEPI3 3905′-GTATCTGCTGAACTCGCTGTTC-3′379
cepR CEPR1 5′-CGGGATCC-1347-GAGAAAGAATGGAACTGCGC-3′1366 55 866
CEPR2 22175′-TCAGCAGAAGCTCGAGCAGAT-3′2197
cepR CEPR1 5′-CGGGATCC-1347-GAGAAAGAATGGAACTGCGC-3′1366 55 494
CEPR3 18455′-ATGAAGCGGCTCAGCGAAT-3′1824
cepR CEPR1 5′-CGGGATCC-1347-GAGAAAGAATGGAACTGCGC-3′1366 55 575
CEPR4 19925′-TTGTTCACGTGGAAGTTGAC-3′1973
bviI BVII1 13415′-CGCAAAGTATCGGCATAAGG-3′1322 55 600
BVII2 8465′-CTGTTCGTCGATCTCGATCCC-3′866
bviR BVIR1 32315′-GGAATTTGACGGTGCGGTCG-3′3212 55 471
BVIR2 27605′-ATGCTGCAGTCCAACTATCC-3′2779
a

PCR conditions were 94°C for 3 min (1 cycle) and 94°C for 1 min, annealing at the indicated temperature for 1 min, and 72°C for 1 min (30 cycles). 

b

Underlined region represents BamHI site.