TABLE 2.
Gene | Primer | Sequence | Annealing temp (°C)a | Amplicon size (bp) |
---|---|---|---|---|
cepI | CEPI1 | 5′-GCGGATCC-121-ACCAGACGCCCATCTACCTGCTTCG-3′134b | 59 | 598 |
CEPI2 | 6995′-GTTACCAGTTACAGGCTCCTC-3′679 | |||
cepI | CEPI1 | 5′-GCGGATCC-121-ACCAGACGCCCATCTACCTGCTTCG-3′134 | 59 | 278 |
CEPI3 | 3905′-GTATCTGCTGAACTCGCTGTTC-3′379 | |||
cepR | CEPR1 | 5′-CGGGATCC-1347-GAGAAAGAATGGAACTGCGC-3′1366 | 55 | 866 |
CEPR2 | 22175′-TCAGCAGAAGCTCGAGCAGAT-3′2197 | |||
cepR | CEPR1 | 5′-CGGGATCC-1347-GAGAAAGAATGGAACTGCGC-3′1366 | 55 | 494 |
CEPR3 | 18455′-ATGAAGCGGCTCAGCGAAT-3′1824 | |||
cepR | CEPR1 | 5′-CGGGATCC-1347-GAGAAAGAATGGAACTGCGC-3′1366 | 55 | 575 |
CEPR4 | 19925′-TTGTTCACGTGGAAGTTGAC-3′1973 | |||
bviI | BVII1 | 13415′-CGCAAAGTATCGGCATAAGG-3′1322 | 55 | 600 |
BVII2 | 8465′-CTGTTCGTCGATCTCGATCCC-3′866 | |||
bviR | BVIR1 | 32315′-GGAATTTGACGGTGCGGTCG-3′3212 | 55 | 471 |
BVIR2 | 27605′-ATGCTGCAGTCCAACTATCC-3′2779 |
PCR conditions were 94°C for 3 min (1 cycle) and 94°C for 1 min, annealing at the indicated temperature for 1 min, and 72°C for 1 min (30 cycles).
Underlined region represents BamHI site.