Table 6.
Effects of experimental diets on plasma alpha-tocopherol and antioxidant activity of enzymes in broilers at 21 days of age 1.
| Item | CONTROL | SPICY | p-Value |
|---|---|---|---|
| α-tocopherol (µg/mL) | 6.09 ± 0.37 | 6.90 ± 0.40 | 0.16 |
| GPx (mU/mL) 2 | 3283 ± 132 | 3329 ± 122 | 0.80 |
| GST (mU/mL) 3 | 27.4 ± 1.17 | 30.4 ± 1.51 | 0.13 |
| SOD (%) 4 | 43.5 ± 7.07 | 39.5 ± 3.61 | 0.59 |
| CAT (U/mL) 5 | 1.54 ± 0.070 | 1.30 ± 0.080 | 0.041 |
1 Data are means ± standard error (n = 12 replicates). 2 One unit of glutathione peroxidase is defined as the amount of enzyme that will cause the oxidation of 1.0 nmol of NADPH to NADP per minute at 25 °C. 3 One unit of glutathione S-transferase will conjugate 1.0 nmol of 1-chloro-2,4-dinitrobenzene with reduced glutathione per minute at 25 °C. 4 One unit of superoxide dismutase is defined as the amount of enzyme needed to exhibit 50% dismutation of the superoxide radical measured in change in absorbance per minute at 25 °C and pH 8.0. 5 One unit of catalase is the amount of enzyme that will cause the formation of 1.0 nmol of formaldehyde per minute at 25 °C.