Figure 4.

Effect of Schot and Spina on the NO production induced by LPS in the culture media of the Wt (A) and Acox1^−/− (B) BV-2 microglial cells assessed by the Griess test. Cells were incubated for 24h with Schot or Spina (1 or 2.5 µM) in the absence or the presence of LPS (1 µg/mL). All values are presented as means ± SD of two independent experiments performed in triplicate, with the statistical significance of the increase in mean signal indicated as ### p ≤ 0.01 compared to LPS and $$$ p ≤ 0.001 and $$ p ≤ 0.01 compared to the different treatments with or without LPS administration. Statistical significance was determined using two-way ANOVA followed by Tukey’s test for multiple comparisons.